A comprehensive map of genome-wide gene regulation in Mycobacterium tuberculosis.

TitleA comprehensive map of genome-wide gene regulation in Mycobacterium tuberculosis.
Publication TypeJournal Article
Year of Publication2015
AuthorsTurkarslan S, Peterson EJR, Rustad TR, Minch KJ, Reiss DJ, Morrison R, Ma S, Price ND, Sherman DR, Baliga NS
JournalSci Data
Volume2
Pagination150010
Date Published2015
ISSN2052-4463
Abstract

Mycobacterium tuberculosis (MTB) is a pathogenic bacterium responsible for 12 million active cases of tuberculosis (TB) worldwide. The complexity and critical regulatory components of MTB pathogenicity are still poorly understood despite extensive research efforts. In this study, we constructed the first systems-scale map of transcription factor (TF) binding sites and their regulatory target proteins in MTB. We constructed FLAG-tagged overexpression constructs for 206 TFs in MTB, used ChIP-seq to identify genome-wide binding events and surveyed global transcriptomic changes for each overexpressed TF. Here we present data for the most comprehensive map of MTB gene regulation to date. We also define elaborate quality control measures, extensive filtering steps, and the gene-level overlap between ChIP-seq and microarray datasets. Further, we describe the use of TF overexpression datasets to validate a global gene regulatory network model of MTB and describe an online source to explore the datasets.

DOI10.1038/sdata.2015.10
Alternate JournalSci Data
PubMed ID25977815
PubMed Central IDPMC4413241
Grant List2P50GM076547 / GM / NIGMS NIH HHS / United States
HHSN272200800059C / / PHS HHS / United States
P50 GM076547 / GM / NIGMS NIH HHS / United States
T32AI007509. / AI / NIAID NIH HHS / United States
U19 AI106761 / AI / NIAID NIH HHS / United States